The pathogen collection consists of ~ 2,000 strains representing recent clinical isolates and multiple engineered isogenic strain panels developed for use in assessing mode-of-action type studies on multiple classes of antibiotic. The former is biased toward bacterial pathogens associated with human device-type (biofilm) infections; whereas the latter includes multiple Gram-positive and Gram-negative organisms bearing combinations of genetic alleles that impart resistance to one or more classes of antibiotic. Additional strain panels including those engineered to systematically lack one or more of all the major drug efflux pumps or transporters in a given organism - Systematic Efflux Knock-Out (SEKO) - are also represented. Model and medically relevant microorganisms represented in this collection include Staphylococcus aureus and S. epidermidis, Pseudomonas aeruginosa, Streptococcus pneumoniae, Haemophilus influenzae and Escherichia coli. However, Mycobacterium bovis BCG, Helicobacter pylori, and Clostridium difficile are also represented.
A second prioritized collection of well characterized strain panels is also available. This subset of the larger collection includes the complete Systematic Efflux Knock-Out (SEKO) strain collection consisting of eighty Pseudomonas aeruginosa PAO1-derivatives, sixteen Streptococcus pneumoniae TIGR4-derivatives, and seven Haemophilus influenzae KW20-derivatives each bearing deletions of putative efflux pumps either alone or in combination. This prioritized collection also contains panels of Staphylococcus aureus, Escherichia coli, Helicobacter pylori, Mycobacterium bovis BCG, and Clostridium difficile engineered for use in assessing the mode-of-action of antibiotics that target RNA polymerase (i.e, rifamycins), DNA gyrase and topoisomerase IV (i.e, quinolones), the ribosome (i.e., oxazolidinones), or other targets (i.e., nitroimidazoles). Additional strains including those useful for the direct purification of RNA polymerase from multiple genera of bacteria are also included.